Description_x000D_ Amino Acid Sequence_x000D_ Glp-Phe-Thr-Asp-Val-Asp-Cys-Ser-Val-Ser-Lys-Glu-Cys-Trp-Ser-Val-Cys-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys-Met-Gly-Lys-Lys-Cys-Arg-Cys-Tyr-Gln [Disulfide Bridges: 7−28; 13−33; 17−35]_x000D_ Application_x000D_ Iberiotoxin, recombinant from Mesobuthus tamulus has been used as an inhibitor of large-conductance calcium-activated K+ channels to study its effect on the NLRP3 (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3) inflammasome activation in bone marrow-derived macrophages (BMDMs). It has also been used to block Ca2+- activated K+ channels to determine interleukin(IL)-1β in BMDCs following inflammasome activation._x000D_ Packaging_x000D_ 5, 10, 100 μg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ The single chain peptide iberiotoxin (IbTX) is a selective and reversible inhibitor of high-conductance calcium-activated potassium channels (PK,Ca). It occurs naturally in the venom of the scorpion Buthus tamulus. IbTX also modulates the binding of charybdotoxin (CbTX) to smooth muscle sarcolemmal membranes in a non-competitive manner. IbTX is similar in size to CbTX and shares considerable sequence homology with CbTX. However, IbTX differs in its overall charge in having one fewer basic and four more acidic residues than CbTX._x000D_ Exhibits the same activity as the natural form of the toxin_x000D_ Features and Benefits_x000D_ This compound is featured on the Potassium Channels page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here._x000D_ Preparation Note_x000D_ Soluble in aqueous buffers. Stock solutions of 1 μM can be aliquoted and stored at -20 °C for up to 3 months.