Description_x000D_ General description_x000D_ IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds._x000D_ Application_x000D_ Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents._x000D_ Packaging_x000D_ 10, 50, 100 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections._x000D_ IgG mediates transplacental transport and participates in the secondary immune response. IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy. It has the longest serum half life._x000D_ Analysis Note_x000D_ Protein concentration should be determined after reconstitution by absorbance at 280 nm (E1%/280 = 14)._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.