Description_x000D_ General description_x000D_ The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray._x000D_ Application_x000D_ Imprint® DNA Modification Kit has been used for bisulfite modification of DNA._x000D_ Features and Benefits_x000D_ • Only 50 picograms of DNA or 20 cells are required_x000D_ • Procedure takes less than 2 hours_x000D_ • Greater than 99% conversion rate_x000D_ • Extremely low degradation_x000D_ • Option of convenient one-step protocol_x000D_ • Consistent and reproducible Bisulfite Modification_x000D_ • Can be used with genomic, endonuclease digested, and FFPE DNA_x000D_ Storage and Stability_x000D_ All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes._x000D_ Legal Information_x000D_ Imprint is a registered trademark of Sigma-Aldrich Co. LLC