Description_x000D_ General description_x000D_ The optimal amount of antibody per ChIP assay should be empirically determined by the end-user depending upon the amount of chromatin used per reaction. We recommend testing 1-5 μg per ChIP assay._x000D_ The tumor suppressor protein p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Wildtype p53 protein is expressed at a low level in normal cells but often mutated and expressed at a high level in a variety of transformed cell lines, where it is believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity._x000D_ Immunogen_x000D_ recombinant human wild type p53 1-5._x000D_ Application_x000D_ Suitable for Chip and western blot._x000D_ Physical form_x000D_ Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative._x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours._x000D_ Disclaimer_x000D_ This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices._x000D_ Legal Information_x000D_ Imprint is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Imprint is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Quality_x000D_ ChIP Validation_x000D_ Other Notes_x000D_ View ChIP Validation Data for lot number: 032M4761