Description_x000D_ General description_x000D_ Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product._x000D_ A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols._x000D_ Application_x000D_ Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:_x000D_ • Applied Biosystems 5700_x000D_ • Applied Biosystems 7000_x000D_ • Applied Biosystems 7300_x000D_ • Applied Biosystems 7700_x000D_ • Applied Biosystems 7900_x000D_ • Applied Biosystems 7900 HT Fast_x000D_ • Applied Biosystems 7900 HT_x000D_ • Applied Biosystems StepOnePlus™_x000D_ • Applied Biosystems StepOne™_x000D_ Features and Benefits_x000D_ • Assay results in as little as 33 minutes_x000D_ • Highly efficient and sensitive real-time PCR results_x000D_ • Little/no optimization required_x000D_ Components_x000D_ 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers._x000D_ 250 reactions* = 2 X 1.25 mL tubes_x000D_ 1250 reactions* = 10 X 1.25 mL tubes_x000D_ 5000 reactions* = 1 X 50 mL tube_x000D_ *number of reactions based on a 20uL volume_x000D_ Legal Information_x000D_ ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ ReadyMix is a trademark of Sigma-Aldrich Co. LLC_x000D_ StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ TaqMan is a registered trademark of Roche Molecular Systems, Inc.