Description General description An antifungal antibiotic that acts as an inhibitor of nuclear export because of its ability to interact with and impair the function of the nuclear export factor CRM1. It is about twice as potent as Leptomycin A (Cat. No. 431051) in inhibiting nucleocytoplasmic translocation of Rev, blocks the Rev-dependent export of mRNA into cytoplasm, and suppresses HIV-1 replication in primary human monocytes (IC50 = 600 pM). In addition, LMB effectively induces the accumulation of p53 in the nucleus of normal fibroblasts as well as protects p53 from Mdm2-mediated ubiquitination and degradation. An unsaturated, branched-chain fatty acid with a terminal lactone ring that acts as a potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Exhibits antibiotic, anti-fungal, and anti-tumor properties and blocks the progression of the cell cycle at the G1 and G2 phases. Shown to inhibit nucleo-cytoplasmic translocation of Rev at nanomolar concentrations and block Rev-dependent export of mRNA into the cytoplasm. Also shown to suppress HIV-1 replication in primary human monocytes (IC50 = 600 pM). Effectively induces the accumulation of p53 in the nucleus of normal fibroblasts and protects p53 from Mdm2-mediated ubiquitination and degradation. Useful for studies involving nuclear localization and protein trafficking in eukaryotic cells. Caution! Product contains volatile liquid. Keep product cold at all times. Caution! Product contains volatile liquid. Keep product cold at all times. Packaging 5 μg in Alu drum Biochem/physiol Actions Cell permeable: no Primary Target HIV-1 replication in primary human monocytes Product does not compete with ATP. Reversible: no Target IC50: 600 pM in suppressing HIV-1 replication in primary human monocytes Packaging Packaged under inert gas Warning Toxicity: Flammable (J) Physical form Supplied at 20 µg/ml (5 µg in 250 µl) in absolute ethanol Reconstitution Following initial use, aliquot and freeze (-70°C). Other Notes Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. Kau, T.R., and Silver, P.A. 2003. Drug Discov. Today8, 78. Menendez, S., et al. 2003. Br. J. Cancer88, 636. Xirodimas, D.P., et al. 2001. Exp. Cell Res.270, 66. Kudo, N., et al. 1999. Proc. Natl. Acad. Sci. USA96, 9112. Lain, S., et al. 1999. Exp. Cell Res.248, 457. Wolff, B., et al. 1997. Chem. Biol.4, 139. Yoshida, M., et al. 1990. Exp. Cell Res.187, 150. Komiyama, K., et al. 1985. J. Antibiot.38, 427.