Description General description Cell-permeable inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells. Causes the accumulation of ubiquitinated proteins in neuronal cells. Prevents NF-κB activation in response to TNFα and okadaic acid through inhibition of IκBα degradation, thereby interfering with induction of iNOS in macrophages. The InSolution Proteasome Inhibitor I controls the biological activity of Proteasome. This small molecule/inhibitor is primarily used for Protease Inhibitors applications. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Primary Target chymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells Product does not compete with ATP. Packaging Packaged under inert gas Warning Toxicity: Irritant (B) Sequence Z-Ile-Glu(OtBu)-Ala-Leu-CHO Physical form A 50 mM (5 mg/162 µl) solution of Proteasome Inhibitor I (Cat. No. 539160) in DMSO. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Other Notes Griscavage, J.M., et al. 1996. Proc. Natl. Acad. Sci. USA93, 3308. Mori, S., et al. 1995. J. Biol. Chem.270, 29447. Figueiredo-Pereira, M.E., et al. 1994. J. Neurochem.63, 1578. Traenckner, E.B., et al. 1994. EMBO J.13, 5433."