Description_x000D_ Packaging_x000D_ 1, 5, 10 g in poly bottle_x000D_ Application_x000D_ IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ._x000D_ Other Notes_x000D_ Non-metabolizable galactose analog._x000D_ Reconstitution_x000D_ A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.