Description_x000D_ Application_x000D_ JumpStart™ AccuTaq™ LA DNA Polymerase has been used in polymerase chain reaction(PCR) for the amplification of soil DNA samples and in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs)._x000D_ JumpStart AccuTaq LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5× of Taq DNA polymerase)._x000D_ Features and Benefits_x000D_ • JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity_x000D_ • Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR_x000D_ • Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA_x000D_ • Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods_x000D_ Packaging_x000D_ Supplied with 10× reaction buffer._x000D_ Unit Definition_x000D_ One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C._x000D_ Other Notes_x000D_ View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart._x000D_ Legal Information_x000D_ Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA._x000D_ AccuTaq is a trademark of Sigma-Aldrich Co. LLC_x000D_ Jacobs is a registered trademark of Jacobs_x000D_ JumpStart is a trademark of Sigma-Aldrich Co. LLC