Description_x000D_ General description_x000D_ JumpStart REDTaq DNA Polymerase is Sigma’s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase._x000D_ • The hot start Taq antibody prevents non-specific product formation._x000D_ • Assembled PCR reactions can be placed at room temperature up to 2 hours._x000D_ • Red tracer allows visible tracking of enzyme addition and reaction mixing._x000D_ • PCR can be directly loaded onto an agarose gel without addition of loading buffers._x000D_ • The red tracer is a tracking dye that run at 125bp on a 1% agarose gel._x000D_ Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies._x000D_ Application_x000D_ JumpStart™ REDTaq® DNA Polymerase has been used in:_x000D_ • polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)_x000D_ • endothelial cells DNA derived from reverse transcribed RNA_x000D_ • leg genomic DNA from cricket flies_x000D_ Features and Benefits_x000D_ • Prevents amplification of nonspecific products, resulting in increased efficiency and higher yields of the desired sequence_x000D_ • No lengthy activation step is required for enzyme activity to be restored_x000D_ • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred_x000D_ • Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye additions_x000D_ Packaging_x000D_ The enzyme is provided with an optimized 10× reaction buffer._x000D_ Unit Definition_x000D_ One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C._x000D_ Other Notes_x000D_ View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart._x000D_ Legal Information_x000D_ Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims._x000D_ JumpStart is a trademark of Sigma-Aldrich Co. LLC_x000D_ REDTaq is a registered trademark of Sigma-Aldrich Co. LLC