Description_x000D_ General description_x000D_ KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product._x000D_ A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification._x000D_ Application_x000D_ Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:_x000D_ • Applied Biosystems® 5700_x000D_ • Applied Biosystems 7000_x000D_ • Applied Biosystems 7300_x000D_ • Applied Biosystems 7700_x000D_ • Applied Biosystems 7900_x000D_ • Applied Biosystems 7900 HT Fast_x000D_ • Applied Biosystems 7900 HT_x000D_ • Applied Biosystems StepOnePlus™_x000D_ • Applied Biosystems StepOne™_x000D_ Features and Benefits_x000D_ • Assay results in as little as 33 minutes_x000D_ • Highly efficient and sensitive real-time PCR results_x000D_ • Little/no optimization required_x000D_ Components_x000D_ 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers_x000D_ Legal Information_x000D_ Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ KiCqStart is a registered trademark of Qiagen Beverly Inc._x000D_ LightCycler is a registered trademark of Roche_x000D_ ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ ReadyMix is a trademark of Sigma-Aldrich Co. LLC_x000D_ Scorpions is a registered trademark of Qiagen Group_x000D_ StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ TaqMan is a registered trademark of Roche Molecular Systems, Inc.