Description_x000D_ General description_x000D_ L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group._x000D_ Application_x000D_ For use in cell culture applications as an antioxidant and to regenerate tocopherol._x000D_ L-ascorbic acid has been used:_x000D_ • in an osteogenic supplement for the growth of cells_x000D_ • as a component in the differentiation media_x000D_ • in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects_x000D_ Biochem/physiol Actions_x000D_ L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.