Description_x000D_ General description_x000D_ L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group._x000D_ Application_x000D_ L-Glutamate Oxidase from Streptomyces sp may be used:_x000D_ • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development_x000D_ • as a component of pencil graphite electrode for biosensing L-glutamate_x000D_ Biochem/physiol Actions_x000D_ L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits._x000D_ Unit Definition_x000D_ One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.