Description_x000D_ General description_x000D_ L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold._x000D_ Application_x000D_ L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA)._x000D_ Packaging_x000D_ 12500 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications._x000D_ Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids._x000D_ Unit Definition_x000D_ One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C._x000D_ Physical form_x000D_ Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5_x000D_ Analysis Note_x000D_ Protein determined by biuret