Description_x000D_ General description_x000D_ Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates._x000D_ Application_x000D_ LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus and soil samples for the isolation of Bacillus spp._x000D_ Features and Benefits_x000D_ Miller’s LB powder provides:_x000D_ • Easy scale-up using larger package sizes_x000D_ • A budget-friendly alternative to liquid_x000D_ • Standard formulation_x000D_ Components_x000D_ 10g/L Tryptone_x000D_ 10g/L NaCl_x000D_ 5g/L Yeast Extract_x000D_ Reconstitution_x000D_ Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).