Description_x000D_ General description_x000D_ Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth._x000D_ Application_x000D_ Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®._x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ Features and Benefits_x000D_ Lennox EZMix™ powder with agar provides:_x000D_ • A budget-friendly alternative to pre-poured plates_x000D_ • Granulated, dust-free format for safer handling and faster mixing_x000D_ • Convenient small package to eliminate weighing_x000D_ • Easy scale-up using larger package sizes_x000D_ • Standard formulation_x000D_ Components_x000D_ 15g/L Agar_x000D_ 10g/L Tryptone_x000D_ 5 g/L Yeast Extract_x000D_ 5 g/L NaCl_x000D_ Reconstitution_x000D_ Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify._x000D_ Legal Information_x000D_ EZMix is a trademark of Sigma-Aldrich Co. LLC_x000D_ Zeocin is a registered trademark of Cayla Sarl