Description_x000D_ General description_x000D_ Luria’s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth._x000D_ Application_x000D_ LB Broth with agar (Luria low salt) has been used for culturing E. coli DH10B cells and in the isolation of diazotrophic bacteria._x000D_ Features and Benefits_x000D_ Luria’s low salt LB powder with agar provides:_x000D_ • Easy scale-up using larger package sizes_x000D_ • A budget-friendly alternative to pre-poured plates_x000D_ • Standard formulation_x000D_ Components_x000D_ 15g/L Agar_x000D_ 10g/L Tryptone_x000D_ 5g/L Yeast Extract_x000D_ 0.5g/L NaCl_x000D_ Reconstitution_x000D_ Add 30.5g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify._x000D_ Legal Information_x000D_ Zeocin is a registered trademark of Cayla Sarl