Description_x000D_ Application_x000D_ Leptin from mouse has been used:_x000D_ • to check the effect of leptin on ovulation for treating infertility in obese mice_x000D_ • to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice_x000D_ • to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin_x000D_ Biochem/physiol Actions_x000D_ Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation._x000D_ Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo._x000D_ Human and mouse leptin share ~84% sequence identity._x000D_ Physical form_x000D_ Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3_x000D_ Analysis Note_x000D_ The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.