Description_x000D_ General description_x000D_ Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms._x000D_ Application_x000D_ Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement._x000D_ Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity._x000D_ Lipase from Candida rugosa has been used:_x000D_ • in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites_x000D_ • in adsorption of proteins in emulsan/alginate beads and in enzyme assays_x000D_ • to catalyse methanolysis and hydrolysis reactions_x000D_ Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel._x000D_ Packaging_x000D_ 1000000, 5000000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis._x000D_ Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate)._x000D_ Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation)._x000D_ Analysis Note_x000D_ Protein determined by biuret.