Description_x000D_ General description_x000D_ Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains._x000D_ Application_x000D_ Lysozyme human has been used:_x000D_ • in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques_x000D_ • in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system_x000D_ • in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)_x000D_ Packaging_x000D_ 1, 10 g in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis._x000D_ Features and Benefits_x000D_ Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%._x000D_ Advantages:_x000D_ • Improves Bacterial Fermentation Efficiency_x000D_ • Greater bacterial lysis activity per mg._x000D_ • 1 gram of delivers more lysis activity than 4 grams of HEWL_x000D_ • Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing_x000D_ • Improved recombinant protein yield compared to mechanical lysis_x000D_ • Reduces cost of goods_x000D_ Packaging_x000D_ Package size based on protein content_x000D_ Unit Definition_x000D_ One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path)._x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer_x000D_ Legal Information_x000D_ Lysobac is a trademark of InVitria