Description_x000D_ General description_x000D_ Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction._x000D_ Application_x000D_ Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts._x000D_ Suitable for the purification of both DNA and protein from bacteria._x000D_ Biochem/physiol Actions_x000D_ Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane._x000D_ The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M)._x000D_ Suitability_x000D_ Tested for suitability with CelLytic™ B in protein extraction from bacteria._x000D_ Physical form_x000D_ Solution in 50% glycerol, 25 mM Sodium Acetate_x000D_ Legal Information_x000D_ CelLytic is a trademark of Sigma-Aldrich Co. LLC