Description_x000D_ General description_x000D_ Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle._x000D_ Mitochondrial Isozyme_x000D_ Application_x000D_ Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens._x000D_ Biochem/physiol Actions_x000D_ Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form._x000D_ The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD_x000D_ Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C._x000D_ Unit Definition_x000D_ One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C._x000D_ Physical form_x000D_ Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5