Description General description Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates. Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates. Packaging 5 μg in Plastic ampoule Warning Toxicity: Standard Handling (A) Unit Definition One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C. Physical form In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5. Reconstitution Following initial thaw, aliquot and freeze (-70°C). Other Notes Lew, J. 2003. Biochemistry42, 849. Johnson, G.L., and Lapadat, R. 2002. Science298, 1911. Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.