Description Specificity Corresponds to the C-terminus of the longer form of BKCa channel a subunit and overlaps by >40 residues with a shorter form. Immunogen A purified fusion protein (GST)1 and a C-terminal part (residues 1097-1196) of mouse Slo (mSlo) alpha subunit of BKCa channel. Epitope: C-terminal sequence SHSSHSSQ Application Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 detects level of Potassium Channel MaxiK & has been published & validated for use in WB. Research Category Neuroscience Research Sub Category Ion Channels & Transporters Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL Dilutions should be made using a carrier protein such as BSA (1-3%). Typical bands detected are ~125kDa in rat membrane preparations. Excellent methods given in Ling, et al (2003) {http://jp.physoc.org/cgi/content/full/553/1/65} Optimal working dilutions must be determined by the end user. Physical form Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). Storage and Stability Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.