Description_x000D_ Amino Acid Sequence_x000D_ Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2_x000D_ General description_x000D_ Chemical structure: peptide_x000D_ Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct haemolytic agent.† The encoded protein containins 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840 Daltons and tetrameric form has molecular weight of approximately 12,500 Daltons._x000D_ Application_x000D_ Melittin from honey bee venom has been used:_x000D_ • In 3-(4, 5-dimethyl thiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay to determine its cytotoxicity effect on the growth of human cell lines._x000D_ • To study the anti-microbial activity of melittin on the growth of Borrelia burgdorferi in in vitro conditions._x000D_ • As a positive control in hemolysis assay and as a cytotoxic agent against HeLa cells._x000D_ Packaging_x000D_ 1, 5, 25 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Melittin acts as an anti-coagulating protein by increasing the time of blood clotting in vitro. Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment._x000D_ Binds calmodulin in a Ca2+-dependent manner; inhibits Na+-K+-ATPase._x000D_ Other Notes_x000D_ The principle hemolytic component of honeybee venom.