Description_x000D_ General description_x000D_ Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence._x000D_ Application_x000D_ Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active. The critical component of nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein)._x000D_ The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase._x000D_ For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page._x000D_ Biochem/physiol Actions_x000D_ Generates Nanodiscs ~9.7 nm in diameter_x000D_ Legal Information_x000D_ Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois._x000D_ • 7,691,414 Membrane scaffold proteins_x000D_ • 7,662,410 Membrane scaffold proteins and embedded membrane proteins_x000D_ • 7,622,437 Tissue factor compositions and methods_x000D_ • 7,592,008 Membrane scaffold proteins_x000D_ • 7,575,763 Membrane scaffold proteins and tethered membrane proteins_x000D_ • 7,083,958 Membrane scaffold proteins_x000D_ • 7,048,949 Membrane scaffold proteins_x000D_ Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois._x000D_ • 7,691,414 Membrane scaffold proteins_x000D_ • 7,662,410 Membrane scaffold proteins and embedded membrane proteins_x000D_ • 7,622,437 Tissue factor compositions and methods_x000D_ • 7,592,008 Membrane scaffold proteins_x000D_ • 7,575,763 Membrane scaffold proteins and tethered membrane proteins_x000D_ • 7,083,958 Membrane scaffold proteins_x000D_ • 7,048,949 Membrane scaffold proteins