Description_x000D_ General description_x000D_ MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes._x000D_ Application_x000D_ MES Hydrate has been used:_x000D_ • As a component of extraction buffer in the extraction of the enzyme BACE1_x000D_ • As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts_x000D_ • As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads_x000D_ Packaging_x000D_ 50, 250 g in poly bottle_x000D_ Preparation Note_x000D_ A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered._x000D_ Storage and Stability_x000D_ Solutions are stable at 2-8 C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown._x000D_ Other Notes_x000D_ Easily compare specifications for MES products with the MES specification table._x000D_ Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.