Description_x000D_ General description_x000D_ MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment._x000D_ The MISSION LentiPlex Mouse shRNA Pooled Library 1.5 includes additional gene and clone coverage from the previously offered LentiPlex 1.0 Pooled Library . With the addition of 1.5, which consists of 4,000+ mouse genes and 10,000+ total clone coverage divided into 3 subpools, the complete whole-genome mouse library complete whole-genome mouse librarynow consists of over 110,000 shRNA constructs from the TRC collection targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The complete library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into sixteen subpools of approximately 6,000-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification._x000D_ For more information including sample data and uses, please visit our LentiPlex Technical Detail Page._x000D_ Other Notes_x000D_ The MISSION LentiPlex Human Pooled shRNA Libraries consist of Catalog Numbers SHPH01, SHPH15, SHPH2, and SHPHLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library._x000D_ Amplification and sequencing primers are also provided for downstream hit identification._x000D_ Deconvolution of shRNA Pools_x000D_ Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen._x000D_ • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample_x000D_ • Comprehensive, reproducible results from pooled shRNA screens_x000D_ • Statistically robust and information-rich data_x000D_ Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi_x000D_ Recommended products_x000D_ Browse additional MISSION shRNA Gene Family Sets in our MISSION shRNA portal._x000D_ Legal Information_x000D_ Lentiviral License Agreement_x000D_ LentiPlex is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ MISSION is a registered trademark of Sigma-Aldrich Co. LLC