Description General description Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use. Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use. Note: 1 mU = 1 milliunit. Packaging 5 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Unit Definition One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0. Physical form In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0. Preparation Note Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Reconstitution Following initial thaw, aliquot and freeze (-70°C). Other Notes Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151. Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414. Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71. Legal Information Brij is a registered trademark of Croda International PLC