Description_x000D_ General description_x000D_ 3-nitrotyrosine (3-NT) is synthesized by the incorporation of nitro triatomic group (-NO2) at the third position of the phenolic ring in the tyrosine residue, in response to the production of peroxynitrite in the oxidative and nitrosative stress._x000D_ Immunogen_x000D_ 3-Nitrotyrosine-KLH._x000D_ Application_x000D_ Monoclonal Anti-3-Nitrotyrosine antibody produced in mouse has been used in western blot analysis and immunohistochemistry._x000D_ Target description_x000D_ Monoclonal Anti-3-Nitrotyrosine recognizes 3-nitrotyrosine in most species. Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling._x000D_ Physical form_x000D_ Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, and 3 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ 3-Nitrotyrosine (3-NT) in tissue extracts is a marker for the determination of protein damage induced by peroxynitrite. 3-NT is used as a biomarker to determine the protein nitration, associated with various disorders. It is implicated in diseases characterized by endothelial dysfunction as in stroke, myocardial infarction, atherosclerosis and diabetes.