Description_x000D_ General description_x000D_ β-Galactosidase is coded by the LacZ gene which helps to transform lactose into galactose._x000D_ β-galactosidase is a ~465 kDa tetrameric protein. Eukaryotic genes are usually cloned into E.coli β-galactosidase (lacZ) gene that results in a fusion hybrid of the desired gene product with the β-galactosidase enzyme . As the amount of the desired gene product in these cloned vector systems corresponds to the amount of β-galactosidase, enzyme assays for β-galactosidase can be utilized to detect the required gene product . Monoclonal anti-β-galactosidase antibodies are specific for β-galactosidase in E.coli and enable the analysis of β-galactosidase fusion proteins encoded by cloned vector systems._x000D_ Specificity_x000D_ The antibody may be used for detection of β-galactosidase expressed by the E. coli lacZ gene encoded in many cloned gene sequences; serves as an indicator for fusion proteins encoded by an inserted DNA sequence._x000D_ Immunogen_x000D_ β-D-galactosidase from E. coli_x000D_ Application_x000D_ Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used for western blotting._x000D_ Monoclonal anti-β-galactosidase antibodies may be used in dot blot (using soluble β-galactosidase), western blot (at a dilution of 1:1,000, using denatured-reduced Escherichia coli β-galactosidase) and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the stepwise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ β-galactosidase catalyses the hydrolysis of lactose to form glucose and galactose. It serves as a reporter enzyme in a number of analysis such as studying the regulation of gene expression, protein function and structure determination, and examining of target gene expression. β-galactosidase is also known to yield coloured products by acting on several substrates. Thus, it is prefered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure, and target gene expression.