Description_x000D_ General description_x000D_ Desmin is the protein subunit of muscle-type intermediate filaments and is found predominantly in skeletal, cardiac, and smooth muscle._x000D_ Monoclonal Anti-Desmin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The desmin protein contains a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. Desmin is is present in the periphery of Z-disk in smooth and striated muscles._x000D_ Specificity_x000D_ The antibody localizes desmin in cultured cells and tissue sections from several species and in tumors derived from muscle tissue (leiomyomas, rhabdomyosarcomas)._x000D_ Immunogen_x000D_ desmin from pig stomach_x000D_ Application_x000D_ Monoclonal Anti-Desmin antibody produced in mouse has been used in:_x000D_ • immunohistochemical staining_x000D_ • immunoblot analyses_x000D_ • immunocytochemistry_x000D_ • immunocytochemical localization of intermediate filaments_x000D_ Monoclonal Anti-Desmin antibody produced in mouse is suitable for indirect immunofluorescence at a working dilution of 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, microarray and western blotting. It may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. It specifically stains desmin when used in immunoblotting assays. It may be used for labelling desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). It was used for immunohistochemical staining of cardiac cells in a study to understand cardiac conduction through engineered tissue. It was used as a primary antibody for the detection of expression of desmin in cultured cells to study the effect of mutation in desmin gene._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Desmin provides mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Mutations in this gene damages the stability of intermediary filaments in cardiomyocytes.