Description_x000D_ Specificity_x000D_ Specifically detects human recombinant and native EDG-6/S1P4 protein (approximately 45 kDa)._x000D_ Immunogen_x000D_ synthetic peptide derived from the C-terminal region of the EDG-6/S1P4 protein._x000D_ Application_x000D_ Anti-S1P4, C-Terminal antibody produced in rabbit is suitable for western blotting and flow cytometry at a working antibody concentration of 5-10μg/mL using RH7777 rat hepatoma cells transfected with EDG-6 protein._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Sphingosine-1-phosphate receptor 4 (S1PR4, EDG-6) is a human gene, which encodes a G protein-coupled receptor and is identified as an orphan G-protein-coupled receptor. It binds the lipid signaling molecule sphingosine 1-phosphate (S1P). EDG-6 is a member of the endothelial differentiation gene (EDG) G protein-coupled receptor family. It is expressed in lymphoid, hematopoietic tissue and in the lung. EDG-6 acts as a high affinity receptor for SPP that couples to a G(i/o) protein leading to the activation of growth-related signaling pathways. It is one of the S1P or lysosphingolipid receptors that couples to phospholipase C-Ca2+ system through pertussis toxin-sensitive G-proteins._x000D_ Analysis Note_x000D_ To detect native EDG-6/S1P4 in cells and tissues, use higher antibody concentrations.