Description_x000D_ General description_x000D_ FKHR belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turn-helix motif. FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB)._x000D_ Monoclonal Anti-FKHR (FOXO1a) (mouse IgG1 isotype) is derived from the FKH117 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human FKHR, conjugated to KLH._x000D_ Immunogen_x000D_ synthetic peptide corresponding to human FKHR (amino acids 636-651), conjugated to KLH._x000D_ Application_x000D_ Monoclonal Anti-FKHR (FOXO1a) antibody is suitable for immunoblot assay to study the down-regulation of both p16INK4A and p14ARF expression, and immunoblot of SKMCs and HSMM cells in a study of stable ectopic expression of PAX3-FKHR in low passage primary human skeletal muscle cells. It is also suitable for microarray and indirect ELISA._x000D_ Monoclonal Anti-FKHR antibody has been used in:_x000D_ • immunoblotting_x000D_ • enzyme linked immunosorbent assay (ELISA)_x000D_ • immunoprecipitation_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Packaging_x000D_ 200 μL in glass bottle_x000D_ Biochem/physiol Actions_x000D_ FKHR plays a key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. It was first identified at chromosomal breakpoints in human tumors and consequently linked to tumorigenesis. The activity of FKHR is regulated by growth factors via the PKB/PI3K pathway. The expression of FKHR is inhibited through phosphorylation by PKB. Within the nucleus, the dephosphorylated FKHRL1 induces expression of target genes such as Fas ligand, and triggers apoptosis. It is involved in apoptosis, glucose homeostasis, cell cycle regulation and as a nuclear receptor co factor. It has been found that FKHR also play a role in endometrial differentiation via the protein kinase A (PKA) pathway.