Description_x000D_ General description_x000D_ The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins._x000D_ Application_x000D_ Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host._x000D_ Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes._x000D_ Browse additional application references in our FLAG® Literature portal._x000D_ Physical form_x000D_ Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide_x000D_ Legal Information_x000D_ ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ FLAG is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Preparation Note_x000D_ Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.