Description_x000D_ General description_x000D_ Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent._x000D_ F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications._x000D_ Method of purification - Protein A_x000D_ Immunogen_x000D_ FLAG; peptide sequence DYKDDDDK_x000D_ Application_x000D_ Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in:_x000D_ • immunoblotting_x000D_ • immunoprecipitation_x000D_ • immunocytochemistry_x000D_ • immunofluorescence_x000D_ • ELISA_x000D_ • chromatin immunoprecipitation_x000D_ • electron microscopy_x000D_ • flow cytometry_x000D_ • supershift assays_x000D_ Browse additional application references in our FLAG® Literature portal._x000D_ Storage and Stability_x000D_ Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended._x000D_ Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications._x000D_ Legal Information_x000D_ ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ FLAG is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Packaging_x000D_ 5 mg in poly bottle_x000D_ Preparation Note_x000D_ Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk