Description_x000D_ Specificity_x000D_ The antibody reacts specifically with L-glutamate (L-glutamic acid), when immobilized with glutaraldehyde. Cross-reaction is observed with Gly-Glu and Asp-Glu. When using the product at the recommended working dilution, in indirect and competitive ELISA, weak cross-reaction may be obtained with D-glutamate, L-glutamine, L-aspartate, D-aspartate, L-asparagine, β-alanine, glycine, 5-aminovaleric acid, γ-aminobutyric acid (GABA) and Gly-Asp. Glutamate is found in cell bodies, axons, and terminals of glutamatergic neurons in the CNS._x000D_ Immunogen_x000D_ L-glutamic acid (Glu)._x000D_ Application_x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immuno-electron microscopy (1 paper)_x000D_ Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:_x000D_ • immunolabeling of glutamate in the lamina of the Drosophila visual system_x000D_ • immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria_x000D_ • immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice_x000D_ • use as a primary antibody to measure the glutamate release in synaptosome preparations_x000D_ It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington’s disease, Alzheimer’s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.