Description_x000D_ General description_x000D_ The gene LEMD3 (LEM domain containing 3) is mapped to human chromosome 12q14. The encoded protein localizes in the inner nuclear membrane. It belongs to the LEM protein family. The protein has nucleoplasmic domains at the carboxy and amino terminals. One nucleoplasmic domain contains LEM motif and the other has binding sites for Smad2 (also referred to as MADH2 (mothers against decapentaplegic homolog 2)) and Smad3._x000D_ Immunogen_x000D_ LEMD3 purified recombinant protein_x000D_ Physical form_x000D_ Solution in Dulbecco′s Modified Eagle′s Medium with 10% fetal calf serum and 15 mM sodium azide._x000D_ Biochem/physiol Actions_x000D_ LEM proteins are mainly involved in gene regulation, chromatin arrangement and regulation of transcription factor. LEMD3 (LEM domain containing 3) interaction with Smad1/2 (also referred to as MADH (mothers against decapentaplegic homolog)) and Samd3 suppresses BMP (bone morphogenic protein) and TGF (transforming growth factor)-β signaling, respectively. It also plays an important role in the regulation of the clock protein, BMAL1 (brain and muscle ARNT-like 1). Absence of LEMD3 activity causes Buschke-Ollendorff syndrome and sclerosing bone dysplasia.