Description_x000D_ General description_x000D_ LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells._x000D_ Immunogen_x000D_ synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2)._x000D_ Application_x000D_ Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended._x000D_ Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A._x000D_ Physical form_x000D_ Solution containing 15 mM sodium azide._x000D_ Biochem/physiol Actions_x000D_ The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.