Description_x000D_ General description_x000D_ Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) (mouse IgG1 isotype) is derived from the ME20.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a human melanoma cell line WM245. The human NGFR p75 has a hydrophobic signal sequence, a single N-linked glycosylation site, four cysteine-rich repeat units of approximately 40 amino acids in the extracellular domain, a serine- and threonine-rich region which might be O-linked glycosylated, a single transmembrane domain, and a 155-amino acid cytoplasmic domain._x000D_ Recognizes the low affinity human NGFR p75 (also known as gp75NGFR , p75NGFR and p75NTR). The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule._x000D_ Specificity_x000D_ Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kDa), also known as gp75NGFR, p75NGFR and p75NTR .The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon , but not with rat, chicken or mouse NGFR._x000D_ Immunogen_x000D_ human melanoma cell line WM245._x000D_ Application_x000D_ Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse has been used in immunoblotting. to added in CB._x000D_ Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) may be used:_x000D_ • for enzyme linked immune sorbent assay (ELISA)_x000D_ • immunoprecipitation, immunoblotting and immunohistochemistry_x000D_ • for immunocytochemistry (acetone- or formalin-fixed, Bouin′s solution - or formalin- fixed paraffin-embedded)_x000D_ • for electron- microscopy radioimmunoassay (RIA) (Kd of 7x10-10 M)_x000D_ • flow cytometry_x000D_ • for the inhibition of binding of NGF to the receptor_x000D_ • for rossette assay_x000D_ • as a cholinergic immunotoxin_x000D_ Physical form_x000D_ The product is supplied as ascites fluid with 15 mM sodium azide as a preservative._x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Nerve Growth Factor Receptor (NGFR) p75 enhances the NGF binding affinity of the proto-oncogene product p140trk and also may modulate the kinase activity of p140trk and play a role in signal transduction. Specifically, neurotrophin binding to NGFR p75 stimulates generation of ceramide, activation and translocation of nuclear factor- κ (NF-κB) to the nucleus, and enhancement of Jun kinase (JNK) activity._x000D_ Neurotrophins (NT) are growth factors that are essential for the development and maintenance of the nervous system. One of the receptors that mediate the effects of NTs is the Nerve Growth Factor Receptor/TNFRSF16 (p75NGFR, p75NTR). NGFR mediates the effects of NGF that are important in the survival and differentiation of neurons and neural crest-derived cells, during embryonic and early postnatal development. Expression of NGFR has been associated with favorable prognosis and survival in breast and pancreatic cancers.