Description_x000D_ General description_x000D_ Monoclonal Anti p27Kip1 (mouse IgG1 isotype) is derived from the DCS-72 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant p27Kip1 protein of rodent origin._x000D_ Specificity_x000D_ Using immunoblotting, some preparations may exhibit higher and lower molecular weight bands. which may represent a polyubiquitinated species and breakdown products of p27Kip1._x000D_ Immunogen_x000D_ recombinant rodent p27Kip1 protein._x000D_ Application_x000D_ Monoclonal Anti-p27Kip1 antibody produced in mouse has been used in immunoblotting, immunofluorescence, immunohistochemistry, immunoprecipitation and immunocytochemistry._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ p27, the product of the kinase inhibition protein1 (kip1) gene, is an inducible inhibitor of cyclin dependent kinase activity. p27Kip1 interacts strongly with D-type cyclins complexed with CDK4 and more weakly with cyclin E and CDK2 complexes. Regulation by p27Kip1 may be an essential step in the pathway that links mitogenic signals to cell cycle progression and may be a key molecular event in the physiological process of cell cycle commitment or passage through the restriction point. Thus, the amount of p27Kip1 increases in quiescent cells and rapidly decreases after stimulation with specific mitogens. Constitutive expression of p27Kip1 in cultured cells causes cell cycle arrest in G1.