Description_x000D_ General description_x000D_ As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP._x000D_ Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse._x000D_ Specificity_x000D_ This antibody is specific for phosphorylated tyrosine both as the free amino acid or when conjugated to carriers such as BSA or KLH_x000D_ Immunogen_x000D_ phosphotyrosine conjugated to BSA_x000D_ Application_x000D_ Monoclonal Anti-Phosphotyrosine antibody produced in mouse has been used in_x000D_ • immunocytochemistry_x000D_ • immunoprecipitation_x000D_ • western blotting_x000D_ • peptide binding studies flow cytometry_x000D_ • enzyme linked immunosorbent assay (ELISA)_x000D_ • radioimmunoassay (RIA)_x000D_ • immunoaffinity isolation_x000D_ Physical form_x000D_ This product is supplied as ascites fluid containing 15 mM sodium azide_x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Phosphotyrosine levels enhanced in cellular proteins leads to activation of several cellular processes mediated by phosphotyrosine kinases. Phosphotyrosine residues that are autophosphorylated by the binding of ligands associated with receptors like epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and insulin receptors, functions as the primary integral component of mitogenic signaling cascade. Phosphotyrosine mediates the activation of T-cell as a result of phosphorylation of tyrosine residues by tyrosine kinases in cytoplasmic domains of CD4 an CD8 which in turn phosphorylates TCR-CD3 complex. Phosphotyrosine activity has also been found in many retroviruses whose oncogenes encodes tyrosine-specific protein kinase._x000D_ Tyrosine residue phosphorylation plays a key role in cell growth and differentiation.