Description_x000D_ General description_x000D_ Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence._x000D_ Immunogen_x000D_ peptide corresponding to amino acids 281-299 of human PINCH-1, conjugated to KLH._x000D_ Application_x000D_ Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in:_x000D_ • enzyme linked immunosorbent assay (ELISA)_x000D_ • immunoblotting_x000D_ • immunoprecipitation_x000D_ • co-immunoprecipitation_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma.