Description_x000D_ General description_x000D_ IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep._x000D_ Immunogen_x000D_ Purified rat IgG_x000D_ Application_x000D_ Monoclonal Anti-Rat IgG2a antibody is suitable for use in indirect ELISA (1:5,000), western blot, and immunocytochemistry._x000D_ Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and slot blot immunoassay._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.