Description_x000D_ Immunogen_x000D_ Amino acids 15-374 of human p84 expressed in E. coli._x000D_ Application_x000D_ Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 μg/mL. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 μg/mL. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher._x000D_ Physical form_x000D_ Phosphate-buffered saline, no preservative added._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies._x000D_ Features and Benefits_x000D_ Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.