Description_x000D_ General description_x000D_ Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms._x000D_ Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor._x000D_ Specificity_x000D_ The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis._x000D_ Immunogen_x000D_ rat tyrosine hydroxylase (TH)._x000D_ Application_x000D_ Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in_x000D_ • western blotting_x000D_ • immunofluorescence labelling_x000D_ • immunohistochemical staining_x000D_ • enzyme linked immunosorbent assay (ELISA)_x000D_ • dot blot_x000D_ • immunoprecipitation_x000D_ • immunocytochemistry_x000D_ Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson’s disease (PD).