Description_x000D_ General description_x000D_ Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology._x000D_ Specificity_x000D_ The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections._x000D_ Immunogen_x000D_ peptide containing the carboxy-terminal amino acids of α-tubulin_x000D_ Application_x000D_ Monoclonal Anti-Tyrosine Tubulin has been used in:_x000D_ • indirect immunofluorescent labelling_x000D_ • immunoblotting technique_x000D_ • immunocytochemical staining_x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.