Description General description Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region. This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific. Immunogen Human Packaging 200 μg in Plastic ampoule Application ELISA (1:500-1:2000, see comments) Packaging Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Physical form In PBS, 1% BSA, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Other Notes Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution. Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: ≤0.1% Human IgG2: ≤1% Human IgG3: 100% Human IgG4: ≤0.1% Human IgA: ≤0.1% Human IgE: ≤0.1% Human IgM: ≤0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG1: <0.001% Human IgG2: <0.001% Human IgG3: 100% Human IgG4: <0.001% Human IgA: <0.001% Human IgE: <0.001% Human IgM: <0.001% Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473. Fasullo, F.J., et al. 1989. Clin. Chem.35, 364. Hamilton, R.G. 1987. Clin. Chem.33, 1707. Jefferis, R., et al. 1986. Immunol. Letters10, 223.