Description_x000D_ General description_x000D_ Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family and have two heavy (H) and two light (L) chain, held together by disulphide linkages. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region._x000D_ Specificity_x000D_ The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study._x000D_ Application_x000D_ Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse has been used in:_x000D_ • enzyme linked immunosorbent assay (ELISA)_x000D_ • imprint immunofixation (IIF)_x000D_ • immunofluorometric assay (IFMA)_x000D_ • hemagglutination (HA)_x000D_ • hemagglutination inhibition (HAI)_x000D_ • particle counting immunoassay(PACIA)_x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Packaging_x000D_ 0.2, 0.5 mL in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Although the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess a "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of human IgG.