Description_x000D_ General description_x000D_ Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse._x000D_ The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein._x000D_ Specificity_x000D_ The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells._x000D_ Immunogen_x000D_ smooth muscle vinculin from chicken gizzard._x000D_ Application_x000D_ Monoclonal Anti-Vinculin antibody produced in mouse has been used in:_x000D_ • western blotting._x000D_ • immunofluorescence._x000D_ • immunocytochemistry._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation._x000D_ Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation._x000D_ Other Notes_x000D_ This product can be found as purified product that was produced using cell culture hybridoma product._x000D_ SAB4200729 Anti-Vinculin antibody, Mouse monoclonal_x000D_ clone VIN-11-5, purified from hybridoma cell culture